فصل الـdna من الخضار او من اي شي

[سونهام يغمور]

First, you need to find something that contains DNA. Since DNA is the blueprint for life, everything living contains DNA.

For this experiment, we like to use green split peas. But there are lots of other DNA sources too, such as:

* Spinach
* Chicken liver
* Strawberries
* Broccoli

Certain sources of DNA should not be used, such as:

* Your family pet, Fido the dog
* Your little sister's big toe
* Bugs you caught in the yard

Start Green Split Peas Step 1 Blender Step 1: Blender Insanity!

Put in a blender:

* 1/2 cup of split peas (100ml)
* 1/8 teaspoon table salt (less than 1ml)
* 1 cup cold water (200ml)

Blend on high for 15 seconds.

The blender separates the pea cells from each other, so you now have a really thin pea-cell soup.
Step 2 Straining Peas Step 2: Soapy Peas

Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).

Add 2 tablespoons liquid detergent (about 30ml) and swirl to mix.

Let the mixture sit for 5-10 minutes.

Pour the mixture into test tubes or other small glass containers, each about 1/3 full.
Why am I adding detergent?
Step 3 Enzymes Step 3: Enzyme Power

Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you'll break up the DNA, making it harder to see.

Use meat tenderizer for enzymes. If you can't find tenderizer, try using pineapple juice or contact lens cleaning solution.
What is an Enzyme?
Step 3 Step 4: Alcohol Separation

Tilt your test tube and slowly pour rubbing alcohol (70-95% isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture. Pour until you have about the same amount of alcohol in the tube as pea mixture.

DNA will rise into the alcohol layer from the pea layer. You can use a wooden stick or other hook to draw the DNA into the alcohol.

Alcohol is less dense than water, so it floats on top. Since two separate layers are formed, all of the grease and the protein that we broke up in the first two steps and the DNA have to decide: "Hmmm...which layer should I go to?"

This is sort of like looking around the room for the most comfortable seat. Some will choose the couch, others might choose the rocking chair.

In this case, the protein and grease parts find the bottom, watery layer the most comfortable place, while the DNA prefers the top, alcohol layer.

DNA is a long, stringy molecule that likes to clump together.